t-dna knockout mutant of acbp1  (Syngenta)

Journal: Plant Physiology

Article Title: Depletion of the Membrane-Associated Acyl-Coenzyme A-Binding Protein ACBP1 Enhances the Ability of Cold Acclimation in Arabidopsis ^{1 [OA]}

doi: 10.1104/pp.109.147066

Figure Lengend Snippet: Changes in PC species of wild-type (Col-0) and ACBP1-overexpressing (ox-1 and ox-2) plants grown at 23°C or CA followed by freezing treatment The values are means ± sd (nmol g −1 dry weight; n = 3). Significant differences ( P < 0.05) from the wild type in the same experiment are indicated in boldface.

Article Snippet: A T-DNA knockout mutant of ACBP1 (designated acbp1 ) from Syngenta (SAIL_653_B06) that had been previously characterized ( Xiao et al., 2008a ) was used to investigate the role of ACBP1 in freezing stress.

Techniques:

Journal: Plant Physiology

Article Title: Depletion of the Membrane-Associated Acyl-Coenzyme A-Binding Protein ACBP1 Enhances the Ability of Cold Acclimation in Arabidopsis ^{1 [OA]}

doi: 10.1104/pp.109.147066

Figure Lengend Snippet: Total amount of each head group class in rosettes of wild-type (Col-0) and ACBP1-overexpressing (ox-1 and ox-2) plants grown at 23°C or CA followed by freezing treatment The values are means ± sd (nmol mg −1 dry weight; n = 3). Significant differences ( P < 0.05) from the wild type in the same experiment are indicated in boldface. DGDG, Digalactosyldiacylglycerol.

Article Snippet: A T-DNA knockout mutant of ACBP1 (designated acbp1 ) from Syngenta (SAIL_653_B06) that had been previously characterized ( Xiao et al., 2008a ) was used to investigate the role of ACBP1 in freezing stress.

Techniques:

Journal: Plant Physiology

Article Title: Depletion of the Membrane-Associated Acyl-Coenzyme A-Binding Protein ACBP1 Enhances the Ability of Cold Acclimation in Arabidopsis ^{1 [OA]}

doi: 10.1104/pp.109.147066

Figure Lengend Snippet: The acbp1 mutant seedlings display enhanced freezing tolerance, while ACBP1-complemented plants show increased freezing sensitivity. A and B, NA and CA 11-d-old wild-type (WT), acbp1 mutant, and ACBP1-complemented (com) seedlings after treatment at −12°C for 1 h. After thawing overnight at 4°C, the plates were transferred to a growth chamber (16-h-light [23°C]/8-h-dark [21°C] photoperiods) for a 7-d recovery before photography. C, Survival rate of NA and CA wild-type, acbp1 mutant, and ACBP1-complemented seedlings shown in A and B. Asterisks denote significant differences from the wild type (** P < 0.01, * P < 0.05). Values are means ± sd(n = 3).

Article Snippet: A T-DNA knockout mutant of ACBP1 (designated acbp1 ) from Syngenta (SAIL_653_B06) that had been previously characterized ( Xiao et al., 2008a ) was used to investigate the role of ACBP1 in freezing stress.

Techniques: Mutagenesis

Journal: Plant Physiology

Article Title: Depletion of the Membrane-Associated Acyl-Coenzyme A-Binding Protein ACBP1 Enhances the Ability of Cold Acclimation in Arabidopsis ^{1 [OA]}

doi: 10.1104/pp.109.147066

Figure Lengend Snippet: The acbp1 mutant plants are more tolerant to freezing stress, while ACBP1-complemented plants show increased freezing sensitivity. A and B, NA and CA 5-week-old wild-type (WT), acbp1 mutant, and ACBP1-complemented (com) plants after freezing treatment at the indicated temperatures. The plants were photographed after thawing overnight at 4°C and recovery in a growth chamber (16-h-light [23°C]/8-h-dark [21°C] photoperiods) for 7 d. C, Electrolyte leakage measurement of NA and CA wild-type, acbp1 mutant, and ACBP1-complemented plants after freezing treatment at the indicated temperatures lasting 1 h followed by thawing at 4°C overnight. Asterisks denote significant differences from the wild type (* P < 0.05). Values are means ± sd(n = 3).

Article Snippet: A T-DNA knockout mutant of ACBP1 (designated acbp1 ) from Syngenta (SAIL_653_B06) that had been previously characterized ( Xiao et al., 2008a ) was used to investigate the role of ACBP1 in freezing stress.

Techniques: Mutagenesis

Journal: Plant Physiology

Article Title: Depletion of the Membrane-Associated Acyl-Coenzyme A-Binding Protein ACBP1 Enhances the Ability of Cold Acclimation in Arabidopsis ^{1 [OA]}

doi: 10.1104/pp.109.147066

Figure Lengend Snippet: ACBP1-overexpressing seedlings show increased sensitivity to freezing stress in comparison with the wild type. A and B, NA and CA 11-d-old wild-type (WT) and ACBP1-overexpressing (ox-1 and ox-2) seedlings after treatment at −12°C for 1 h. Plates were thawed overnight at 4°C following a 7-d recovery in a growth chamber (16-h-light [23°C]/8-h-dark [21°C] photoperiods) before photography. C, Survival rate of NA and CA wild-type and ACBP1-overexpressing seedlings after freezing displayed in A and B. Asterisks denote significant differences from the wild type (** P < 0.01). Values are means ± sd(n = 3).

Article Snippet: A T-DNA knockout mutant of ACBP1 (designated acbp1 ) from Syngenta (SAIL_653_B06) that had been previously characterized ( Xiao et al., 2008a ) was used to investigate the role of ACBP1 in freezing stress.

Techniques: Comparison

Journal: Plant Physiology

Article Title: Depletion of the Membrane-Associated Acyl-Coenzyme A-Binding Protein ACBP1 Enhances the Ability of Cold Acclimation in Arabidopsis ^{1 [OA]}

doi: 10.1104/pp.109.147066

Figure Lengend Snippet: ACBP1-overexpressing plants are more sensitive to freezing stress in comparison with the wild type. A and B, NA and CA 5-week-old wild-type (WT) and ACBP1-overexpressing (ox-1 and ox-2) plants after freezing treatment at the indicated temperatures. Plants were photographed after a 7-d recovery in a growth chamber (16-h-light [23°C]/8-h-dark [21°C] photoperiods). C, Electrolyte leakage of NA and CA wild-type and ACBP1-overexpressing plants after freezing treatment at the indicated temperatures lasting 1 h followed by thawing at 4°C overnight. Asterisks denote significant differences from the wild type (* P < 0.05). Values are means ± sd(n = 3).

Article Snippet: A T-DNA knockout mutant of ACBP1 (designated acbp1 ) from Syngenta (SAIL_653_B06) that had been previously characterized ( Xiao et al., 2008a ) was used to investigate the role of ACBP1 in freezing stress.

Techniques: Comparison

Journal: Plant Physiology

Article Title: Depletion of the Membrane-Associated Acyl-Coenzyme A-Binding Protein ACBP1 Enhances the Ability of Cold Acclimation in Arabidopsis ^{1 [OA]}

doi: 10.1104/pp.109.147066

Figure Lengend Snippet: The expression of ACBP1, COR genes, PLDα1, and PLDδ in wild-type (WT), acbp1 mutant, and ACBP1-overexpressing (ox-1 and ox-2) plants. A, Cold induction of ACBP1 expression. Total RNA (20 μg lane−1) was isolated from wild-type Arabidopsis rosettes at the indicated hours (h) after treatment. B, Total RNA (20 μg lane−1) was prepared from rosettes of wild-type, acbp1 mutant, and ACBP1-overexpressing plants before (NA) or after (CA) 3 d of cold acclimation at 4°C. The blots were hybridized with digoxigenin-labeled probes for COR6.6, COR47, and ACBP1. C, The transcript levels of PLDα1 and PLDδ in the wild type, acbp1, and ACBP1 overexpressors. Total RNA (20 μg lane−1) was prepared from wild-type, acbp1, ox-1, and ox-2 rosettes harvested before (NA) or after (CA) 3 d of cold acclimation, followed by freezing at −8°C for 1 h (F) and recovery (R; the temperature was raised to 4°C at 1°C h−1 and held at 4°C for 12 h before sampling). All bottom panels show ethidium bromide-stained rRNA, indicating the relative amounts of total RNA loaded per lane.

Article Snippet: A T-DNA knockout mutant of ACBP1 (designated acbp1 ) from Syngenta (SAIL_653_B06) that had been previously characterized ( Xiao et al., 2008a ) was used to investigate the role of ACBP1 in freezing stress.

Techniques: Expressing, Mutagenesis, Isolation, Labeling, Sampling, Staining

Journal: Plant Physiology

Article Title: Depletion of the Membrane-Associated Acyl-Coenzyme A-Binding Protein ACBP1 Enhances the Ability of Cold Acclimation in Arabidopsis ^{1 [OA]}

doi: 10.1104/pp.109.147066

Figure Lengend Snippet: Total amount of each head group class in rosettes of wild-type (Col-0), acbp1 mutant, and ACBP1-complemented (com) plants grown at 23°C or CA followed by freezing treatment The values are means ± sd (nmol mg −1 dry weight; n = 3). Significant differences ( P < 0.05) from the wild type in the same experiment are indicated in boldface. DGDG, Digalactosyldiacylglycerol.

Article Snippet: A T-DNA knockout mutant of ACBP1 (designated acbp1 ) from Syngenta (SAIL_653_B06) that had been previously characterized ( Xiao et al., 2008a ) was used to investigate the role of ACBP1 in freezing stress.

Techniques: Mutagenesis

Journal: Plant Physiology

Article Title: Depletion of the Membrane-Associated Acyl-Coenzyme A-Binding Protein ACBP1 Enhances the Ability of Cold Acclimation in Arabidopsis ^{1 [OA]}

doi: 10.1104/pp.109.147066

Figure Lengend Snippet: Changes in PA species of wild-type (Col-0) and ACBP1-overexpressing (ox-1 and ox-2) plants grown at 23°C or CA followed by freezing treatment The values are means ± sd (nmol g −1 dry weight; n = 3). Significant differences ( P < 0.05) from the wild type in the same experiment are indicated in boldface.

Article Snippet: A T-DNA knockout mutant of ACBP1 (designated acbp1 ) from Syngenta (SAIL_653_B06) that had been previously characterized ( Xiao et al., 2008a ) was used to investigate the role of ACBP1 in freezing stress.

Techniques:

Journal: Plant Physiology

Article Title: Depletion of the Membrane-Associated Acyl-Coenzyme A-Binding Protein ACBP1 Enhances the Ability of Cold Acclimation in Arabidopsis ^{1 [OA]}

doi: 10.1104/pp.109.147066

Figure Lengend Snippet: Changes in PA species of wild-type (Col-0), acbp1 mutant, and ACBP1-complemented (com) plants grown at 23°C or CA followed by freezing treatment The values are means ± sd (nmol g −1 dry weight; n = 3). Significant differences ( P < 0.05) from the wild type in the same experiment are indicated in boldface.

Article Snippet: A T-DNA knockout mutant of ACBP1 (designated acbp1 ) from Syngenta (SAIL_653_B06) that had been previously characterized ( Xiao et al., 2008a ) was used to investigate the role of ACBP1 in freezing stress.

Techniques: Mutagenesis

Journal: Plant Physiology

Article Title: Depletion of the Membrane-Associated Acyl-Coenzyme A-Binding Protein ACBP1 Enhances the Ability of Cold Acclimation in Arabidopsis ^{1 [OA]}

doi: 10.1104/pp.109.147066

Figure Lengend Snippet: Changes in PC species of wild-type (Col-0), acbp1 mutant, and ACBP1-complemented (com) plants grown at 23°C or CA followed by freezing treatment The values are means ± sd (nmol g −1 dry weight; n = 3). Significant differences ( P < 0.05) from the wild type in the same experiment are indicated in boldface.

Article Snippet: A T-DNA knockout mutant of ACBP1 (designated acbp1 ) from Syngenta (SAIL_653_B06) that had been previously characterized ( Xiao et al., 2008a ) was used to investigate the role of ACBP1 in freezing stress.

Techniques: Mutagenesis

Journal: Plant Physiology

Article Title: Depletion of the Membrane-Associated Acyl-Coenzyme A-Binding Protein ACBP1 Enhances the Ability of Cold Acclimation in Arabidopsis ^{1 [OA]}

doi: 10.1104/pp.109.147066

Figure Lengend Snippet: Changes in soluble sugar (A) and Pro (B) after cold acclimation of wild-type (WT), acbp1 mutant, ACBP1-complemented (com), and ACBP1-overexpressing (ox-1 and ox-2) plants. Values are means ± sd(n = 3).

Article Snippet: A T-DNA knockout mutant of ACBP1 (designated acbp1 ) from Syngenta (SAIL_653_B06) that had been previously characterized ( Xiao et al., 2008a ) was used to investigate the role of ACBP1 in freezing stress.

Techniques: Mutagenesis

Journal: Plant Physiology

Article Title: Depletion of the Membrane-Associated Acyl-Coenzyme A-Binding Protein ACBP1 Enhances the Ability of Cold Acclimation in Arabidopsis ^{1 [OA]}

doi: 10.1104/pp.109.147066

Figure Lengend Snippet: Interaction of recombinant ACBP1 with PA. A, Diagram of lipid species on membrane lipid strips (Echelon Biosciences). B, Binding of full-length (rACBP1) and deletion mutant (rACBP1 Δ ACB) ACBP1 to lipids on membrane lipid strips in A. The strips were incubated with 1 μ g mL−1 purified rACBP1 or rACBP1 Δ ACB protein, and their binding was detected by immunoblotting with HRP-conjugated anti-penta-His antibodies. C, rACBP1/PA binding on filters. Serial concentrations (0, 6, 12.5, 25.0, 32.5, and 50 μm) of 16:0-PA were spotted onto nitrocellulose and incubated with 1 μ g mL−1 purified rACBP1 protein. The rACBP1/PA binding was detected by immunoblotting with HRP-conjugated anti-penta-His antibodies. D, Effect of PA acyl species on rACBP1/PA binding. Twenty micromolar lipids (16:0-PA, 18:0-PA, and18:1-PA) spotted onto nitrocellulose were incubated with 1 μ g mL−1 purified rACBP1 protein. The rACBP1/PA binding was detected by immunoblotting with HRP-conjugated anti-penta-His antibodies. DAG, Diacylglycerol; PtdIns(4)P, phosphatidylinositol 4-phosphate; PtdIns(4,5)P2, phosphatidylinositol 4,5-bisphosphate; PtdIns(3,4,5)P3, phosphatidylinositol 3,4,5-trisphosphate; sulfatide, 3-sulfogalactosylceramide.

Article Snippet: A T-DNA knockout mutant of ACBP1 (designated acbp1 ) from Syngenta (SAIL_653_B06) that had been previously characterized ( Xiao et al., 2008a ) was used to investigate the role of ACBP1 in freezing stress.

Techniques: Recombinant, Membrane, Binding Assay, Mutagenesis, Incubation, Purification, Western Blot